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u87 mg rfp  (ATCC)


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    ATCC u87 mg rfp
    Knock-out of CHI3L1 in <t>U87-MG</t> glioma cells results in gross changes in the expression of genes involved in ECM re-organization and immunosuppression. a Experimental pipeline of generating CHI3L1 <t>knock-out</t> <t>in</t> <t>U87-MG-RFP</t> (U87) human glioblastoma cells using CRISPR/Cas9 technology. b Assessment of CHI3L1 expression on protein level in normal human astrocytes (NHA), two human glioma cell lines (U251 and U87-MG) and one human primary GBM cells (WG9); mean ± SD is presented; statistical analysis by one-way ANOVA, Tukey’s multiple comparison test; **** P ≤ 0.0001. c Cancer Dependency Map (DepMap, Broad Institute) plot of “gene effect” of tested human glioma cell lines. The higher the gene effect, the lower the impact of CRISPR-mediated knock-out of CHI3L1 on the viability of the cells. d-e Validation of CHI3L1 knock-out in U87 cells at mRNA (d) and protein level (e) in three independent passages; mean ± SD is presented; statistical analysis by unpaired t-test; ** P ≤ 0.01 (d); one-sample t-test with hypothetical value of 100% (e). ** P ≤ 0.01. f-h Comparative results of RNA sequencing between WT and CHI3L1 KO U87-MG cells. f Venn diagrams of differentially expressed genes (DEGs) for up- and down-regulated (upper and lower panel, respectively) genes between two CHI3L1 KO clones as compared to WT control. g Volcano plot of DEGs in the CHI3L1 KO clone 1 (KO1) compared to WT control. h Z-score heatmap of the REACTOME pathway enrichment analysis for WT, CHI3L1 KO1 and KO2 cells. Gene clusters indicated with color bars to the left. i . Scatter plots of protein expression from the Brain Protein Atlas study representing positive correlation between CHI3L1 and EFEMP1, ANXA1, NPC2, SERPINH1 and HLA-DRA proteins
    U87 Mg Rfp, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u87+mg+rfp/U-87+MG/pmc12892814-53-5-6
    Average 99 stars, based on 10558 article reviews
    u87 mg rfp - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "Chitinase-3-like protein 1 depletion in glioma cells alters tumor microenvironment and normalizes neovasculature in human glioma xenografts"

    Article Title: Chitinase-3-like protein 1 depletion in glioma cells alters tumor microenvironment and normalizes neovasculature in human glioma xenografts

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-025-02636-8

    Knock-out of CHI3L1 in U87-MG glioma cells results in gross changes in the expression of genes involved in ECM re-organization and immunosuppression. a Experimental pipeline of generating CHI3L1 knock-out in U87-MG-RFP (U87) human glioblastoma cells using CRISPR/Cas9 technology. b Assessment of CHI3L1 expression on protein level in normal human astrocytes (NHA), two human glioma cell lines (U251 and U87-MG) and one human primary GBM cells (WG9); mean ± SD is presented; statistical analysis by one-way ANOVA, Tukey’s multiple comparison test; **** P ≤ 0.0001. c Cancer Dependency Map (DepMap, Broad Institute) plot of “gene effect” of tested human glioma cell lines. The higher the gene effect, the lower the impact of CRISPR-mediated knock-out of CHI3L1 on the viability of the cells. d-e Validation of CHI3L1 knock-out in U87 cells at mRNA (d) and protein level (e) in three independent passages; mean ± SD is presented; statistical analysis by unpaired t-test; ** P ≤ 0.01 (d); one-sample t-test with hypothetical value of 100% (e). ** P ≤ 0.01. f-h Comparative results of RNA sequencing between WT and CHI3L1 KO U87-MG cells. f Venn diagrams of differentially expressed genes (DEGs) for up- and down-regulated (upper and lower panel, respectively) genes between two CHI3L1 KO clones as compared to WT control. g Volcano plot of DEGs in the CHI3L1 KO clone 1 (KO1) compared to WT control. h Z-score heatmap of the REACTOME pathway enrichment analysis for WT, CHI3L1 KO1 and KO2 cells. Gene clusters indicated with color bars to the left. i . Scatter plots of protein expression from the Brain Protein Atlas study representing positive correlation between CHI3L1 and EFEMP1, ANXA1, NPC2, SERPINH1 and HLA-DRA proteins
    Figure Legend Snippet: Knock-out of CHI3L1 in U87-MG glioma cells results in gross changes in the expression of genes involved in ECM re-organization and immunosuppression. a Experimental pipeline of generating CHI3L1 knock-out in U87-MG-RFP (U87) human glioblastoma cells using CRISPR/Cas9 technology. b Assessment of CHI3L1 expression on protein level in normal human astrocytes (NHA), two human glioma cell lines (U251 and U87-MG) and one human primary GBM cells (WG9); mean ± SD is presented; statistical analysis by one-way ANOVA, Tukey’s multiple comparison test; **** P ≤ 0.0001. c Cancer Dependency Map (DepMap, Broad Institute) plot of “gene effect” of tested human glioma cell lines. The higher the gene effect, the lower the impact of CRISPR-mediated knock-out of CHI3L1 on the viability of the cells. d-e Validation of CHI3L1 knock-out in U87 cells at mRNA (d) and protein level (e) in three independent passages; mean ± SD is presented; statistical analysis by unpaired t-test; ** P ≤ 0.01 (d); one-sample t-test with hypothetical value of 100% (e). ** P ≤ 0.01. f-h Comparative results of RNA sequencing between WT and CHI3L1 KO U87-MG cells. f Venn diagrams of differentially expressed genes (DEGs) for up- and down-regulated (upper and lower panel, respectively) genes between two CHI3L1 KO clones as compared to WT control. g Volcano plot of DEGs in the CHI3L1 KO clone 1 (KO1) compared to WT control. h Z-score heatmap of the REACTOME pathway enrichment analysis for WT, CHI3L1 KO1 and KO2 cells. Gene clusters indicated with color bars to the left. i . Scatter plots of protein expression from the Brain Protein Atlas study representing positive correlation between CHI3L1 and EFEMP1, ANXA1, NPC2, SERPINH1 and HLA-DRA proteins

    Techniques Used: Knock-Out, Expressing, CRISPR, Comparison, Biomarker Discovery, RNA Sequencing, Clone Assay, Control

    Related Articles

    Cell Culture:

    Article Title: Chitinase-3-like protein 1 depletion in glioma cells restricts myeloid cell infiltration and normalizes neovasculature in human glioma xenografts
    Article Snippet: RNAs from normal brains were purchased from: Agilent 540005 (lot 0006127195, Female 66 years), Biochain R1234035-50 (lot B304105, Male 25 years), Human Brain Reference RNA AM6050 (lot 1207015, mix of RNA, male and female donors). .. Human cell lines and cell culture Human glioblastoma cell lines LN229, U87-MG, U87-MG-RFP (ATCC, Manassas, VA) and WG9[25] were cultured in Dulbecco’s modi ed Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, MD, USA) and antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin). .. Normal human astrocytes (NHA; Lonza, Cologne, Germany) were cultured in dedicated Clonetics media (Lonza, Walkersville, MD, USA) in CO2/air (5%/95%) at 37°C (Heraeus, Hanau, Germany).

    Article Title: Chitinase-3-like protein 1 depletion in glioma cells alters tumor microenvironment and normalizes neovasculature in human glioma xenografts.
    Article Snippet: RNAs from normal brains were purchased from: Agilent 540005 (lot 0006127195, Female 66 years), Biochain R1234035-50 (lot B304105, Male 25 years), Human Brain Reference RNA AM6050 (lot 1207015, mix of RNA, male and female donors). .. Human cell lines and cell culture Human glioblastoma cell lines U251, U87-MG-RFP (ATCC, Manassas, VA) and GBM patientderived WG9 cell cultures [27] were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, MD, USA) and antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin). .. Normal human astrocytes (NHA; Lonza, Cologne, Germany) were cultured in dedicated Clonetics media (Lonza, Walkersville, MD, USA) in CO2/air (5%/95%) at 37°C (Heraeus, Hanau, Germany).

    Modification:

    Article Title: Chitinase-3-like protein 1 depletion in glioma cells alters tumor microenvironment and normalizes neovasculature in human glioma xenografts
    Article Snippet: RNAs from normal brains were purchased from: Agilent 540,005 (lot 0006127195, Female 66 years), Biochain R1234035-50 (lot B304105, Male 25 years), Human Brain Reference RNA AM6050 (lot 1207015, mix of RNA, male and female donors). .. Human glioblastoma cell lines U251, U87-MG-RFP (ATCC, Manassas, VA) and GBM patient-derived WG9 cell cultures [ ] were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, MD, USA) and antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin). .. Normal human astrocytes (NHA; Lonza, Cologne, Germany) were cultured in dedicated Clonetics media (Lonza, Walkersville, MD, USA) in CO 2 /air (5%/95%) at 37 °C (Heraeus, Hanau, Germany).

    Article Title: Chitinase-3-like protein 1 depletion in glioma cells alters tumor microenvironment and normalizes neovasculature in human glioma xenografts.
    Article Snippet: RNAs from normal brains were purchased from: Agilent 540005 (lot 0006127195, Female 66 years), Biochain R1234035-50 (lot B304105, Male 25 years), Human Brain Reference RNA AM6050 (lot 1207015, mix of RNA, male and female donors). .. Human cell lines and cell culture Human glioblastoma cell lines U251, U87-MG-RFP (ATCC, Manassas, VA) and GBM patientderived WG9 cell cultures [27] were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, MD, USA) and antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin). .. Normal human astrocytes (NHA; Lonza, Cologne, Germany) were cultured in dedicated Clonetics media (Lonza, Walkersville, MD, USA) in CO2/air (5%/95%) at 37°C (Heraeus, Hanau, Germany).



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    Image Search Results


    Knock-out of CHI3L1 in U87-MG glioma cells results in gross changes in the expression of genes involved in ECM re-organization and immunosuppression. a Experimental pipeline of generating CHI3L1 knock-out in U87-MG-RFP (U87) human glioblastoma cells using CRISPR/Cas9 technology. b Assessment of CHI3L1 expression on protein level in normal human astrocytes (NHA), two human glioma cell lines (U251 and U87-MG) and one human primary GBM cells (WG9); mean ± SD is presented; statistical analysis by one-way ANOVA, Tukey’s multiple comparison test; **** P ≤ 0.0001. c Cancer Dependency Map (DepMap, Broad Institute) plot of “gene effect” of tested human glioma cell lines. The higher the gene effect, the lower the impact of CRISPR-mediated knock-out of CHI3L1 on the viability of the cells. d-e Validation of CHI3L1 knock-out in U87 cells at mRNA (d) and protein level (e) in three independent passages; mean ± SD is presented; statistical analysis by unpaired t-test; ** P ≤ 0.01 (d); one-sample t-test with hypothetical value of 100% (e). ** P ≤ 0.01. f-h Comparative results of RNA sequencing between WT and CHI3L1 KO U87-MG cells. f Venn diagrams of differentially expressed genes (DEGs) for up- and down-regulated (upper and lower panel, respectively) genes between two CHI3L1 KO clones as compared to WT control. g Volcano plot of DEGs in the CHI3L1 KO clone 1 (KO1) compared to WT control. h Z-score heatmap of the REACTOME pathway enrichment analysis for WT, CHI3L1 KO1 and KO2 cells. Gene clusters indicated with color bars to the left. i . Scatter plots of protein expression from the Brain Protein Atlas study representing positive correlation between CHI3L1 and EFEMP1, ANXA1, NPC2, SERPINH1 and HLA-DRA proteins

    Journal: Cell Communication and Signaling : CCS

    Article Title: Chitinase-3-like protein 1 depletion in glioma cells alters tumor microenvironment and normalizes neovasculature in human glioma xenografts

    doi: 10.1186/s12964-025-02636-8

    Figure Lengend Snippet: Knock-out of CHI3L1 in U87-MG glioma cells results in gross changes in the expression of genes involved in ECM re-organization and immunosuppression. a Experimental pipeline of generating CHI3L1 knock-out in U87-MG-RFP (U87) human glioblastoma cells using CRISPR/Cas9 technology. b Assessment of CHI3L1 expression on protein level in normal human astrocytes (NHA), two human glioma cell lines (U251 and U87-MG) and one human primary GBM cells (WG9); mean ± SD is presented; statistical analysis by one-way ANOVA, Tukey’s multiple comparison test; **** P ≤ 0.0001. c Cancer Dependency Map (DepMap, Broad Institute) plot of “gene effect” of tested human glioma cell lines. The higher the gene effect, the lower the impact of CRISPR-mediated knock-out of CHI3L1 on the viability of the cells. d-e Validation of CHI3L1 knock-out in U87 cells at mRNA (d) and protein level (e) in three independent passages; mean ± SD is presented; statistical analysis by unpaired t-test; ** P ≤ 0.01 (d); one-sample t-test with hypothetical value of 100% (e). ** P ≤ 0.01. f-h Comparative results of RNA sequencing between WT and CHI3L1 KO U87-MG cells. f Venn diagrams of differentially expressed genes (DEGs) for up- and down-regulated (upper and lower panel, respectively) genes between two CHI3L1 KO clones as compared to WT control. g Volcano plot of DEGs in the CHI3L1 KO clone 1 (KO1) compared to WT control. h Z-score heatmap of the REACTOME pathway enrichment analysis for WT, CHI3L1 KO1 and KO2 cells. Gene clusters indicated with color bars to the left. i . Scatter plots of protein expression from the Brain Protein Atlas study representing positive correlation between CHI3L1 and EFEMP1, ANXA1, NPC2, SERPINH1 and HLA-DRA proteins

    Article Snippet: Human glioblastoma cell lines U251, U87-MG-RFP (ATCC, Manassas, VA) and GBM patient-derived WG9 cell cultures [ ] were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, MD, USA) and antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin).

    Techniques: Knock-Out, Expressing, CRISPR, Comparison, Biomarker Discovery, RNA Sequencing, Clone Assay, Control